Farre Lab:Research: Difference between revisions
Eva M Farre (talk | contribs) No edit summary |
Eva M Farre (talk | contribs) No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 27: | Line 27: | ||
'''Nannochloropsis oceanica''' | '''Nannochloropsis oceanica''' | ||
Nannochloropsis species are small unicellular alga with a diameter of about 2 μm. Marine Nannochloropsis species are used as a source of fish food and omega-3 fatty acids (Adarme-Vega et al., 2012). Due to their high lipid content, which is particularly elevated under nitrogen deprivation, these species have been considered as a potential source of biofuels (Hu and Gao, 2003; Rodolfi et al., 2009; Van Vooren et al., 2012; Xu et al., 2004). The genomes of two Nannochloropsis species have been recently sequenced (Jinkerson et al., 2012; Pan et al., 2011; Radakovits et al., 2012; Vieler et al., 2012). Both species have a small genome of ~30 Mb, containing ~9,000-12,000 genes, similar to the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana (Armbrust et al., 2004; Bowler et al., 2008). Current research suggests that Nannochloropsis species are haploid and homologous gene replacement has been recently reported (Kilian et al., 2011; Pan et al., 2011). Cell division and lipid content are strongly diurnally regulated in Nannochloropsis (Fábregas et al., 2002; Sukenik and Carmeli, 1990). | Nannochloropsis species are small unicellular alga with a diameter of about 2 μm. Marine Nannochloropsis species are used as a source of fish food and omega-3 fatty acids (Adarme-Vega et al., 2012). Due to their high lipid content, which is particularly elevated under nitrogen deprivation, these species have been considered as a potential source of biofuels (Hu and Gao, 2003; Rodolfi et al., 2009; Van Vooren et al., 2012; Xu et al., 2004). The genomes of two Nannochloropsis species have been recently sequenced (Jinkerson et al., 2012; Pan et al., 2011; Radakovits et al., 2012; Vieler et al., 2012). Both species have a small genome of ~30 Mb, containing ~9,000-12,000 genes, similar to the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana (Armbrust et al., 2004; Bowler et al., 2008). Current research suggests that Nannochloropsis species are haploid and homologous gene replacement has been recently reported (Kilian et al., 2011; Pan et al., 2011). Cell division and lipid content are strongly diurnally regulated in Nannochloropsis (Fábregas et al., 2002; Sukenik and Carmeli, 1990). | ||
Clocks have been studied in cyanobacteria, plants, green alga, fungi and animals (Figure 3), however, the mechanisms underlying circadian rhythms in stramenopiles including Nannochloropsis are currently unknown. We have developed circadian reporter lines in different Nannochloropsis species and are currently characterizing their circadian rhythms and searching for clock components. | |||
[[Image:Tree_V2.jpg | center |600 px| Figure 2]] | |||
'''Figure 3.''' Overview of the presence of circadian oscillators in different taxa. Taxa in which circadian rhythms have been detected are in italics. Trc/Trl indicates the presence of a characterized transcriptional/translational circadian clock and the main components are indicated. Trc-less indicates the presence of oscillations of peroxiredoxin oxidation in the absence of transcription (PRX-ox). The green and red circles indicate secondary endosymbiotic events involving green and red alga respectively. Tree is based on tolweb.org. | |||
<h2><font style="color:#006400;">Bibliography</font></h2> | <h2><font style="color:#006400;">Bibliography</font></h2> | ||
Revision as of 12:22, 27 July 2015
|
|
|
|
Circadian clocks
 Circadian systems can be thought of consisting of 3 parts (Figure 1). The imput pathways are involved in the entrainment or reprograming of the central oscillator which is the core of the circadian system. In turn this molecular self-sustained oscillator regulates the different physiological processes by regulating output pathways.
Arabidopsis thaliana We focus our work on the role of the PSEUDO-RESPONSE REGULATORS (PRR)(Farre and Liu, 2013). These proteins are not only involved in the regulation of the Arabidopsis circadian oscillator (Figure 2) but are also involved in the direct regulation of physiological processes (Huang et al., 2012; Liu et al., 2013; Nakamichi et al., 2012).
 Figure 2. Current status of the Arabidopsis circadian clock (2014)
Nannochloropsis species are small unicellular alga with a diameter of about 2 μm. Marine Nannochloropsis species are used as a source of fish food and omega-3 fatty acids (Adarme-Vega et al., 2012). Due to their high lipid content, which is particularly elevated under nitrogen deprivation, these species have been considered as a potential source of biofuels (Hu and Gao, 2003; Rodolfi et al., 2009; Van Vooren et al., 2012; Xu et al., 2004). The genomes of two Nannochloropsis species have been recently sequenced (Jinkerson et al., 2012; Pan et al., 2011; Radakovits et al., 2012; Vieler et al., 2012). Both species have a small genome of ~30 Mb, containing ~9,000-12,000 genes, similar to the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana (Armbrust et al., 2004; Bowler et al., 2008). Current research suggests that Nannochloropsis species are haploid and homologous gene replacement has been recently reported (Kilian et al., 2011; Pan et al., 2011). Cell division and lipid content are strongly diurnally regulated in Nannochloropsis (Fábregas et al., 2002; Sukenik and Carmeli, 1990). Clocks have been studied in cyanobacteria, plants, green alga, fungi and animals (Figure 3), however, the mechanisms underlying circadian rhythms in stramenopiles including Nannochloropsis are currently unknown. We have developed circadian reporter lines in different Nannochloropsis species and are currently characterizing their circadian rhythms and searching for clock components.  Figure 3. Overview of the presence of circadian oscillators in different taxa. Taxa in which circadian rhythms have been detected are in italics. Trc/Trl indicates the presence of a characterized transcriptional/translational circadian clock and the main components are indicated. Trc-less indicates the presence of oscillations of peroxiredoxin oxidation in the absence of transcription (PRX-ox). The green and red circles indicate secondary endosymbiotic events involving green and red alga respectively. Tree is based on tolweb.org. Bibliography
|
