Feruloyl Esterase Protocols

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Introduction

These are methods to screen for and assay Ferulic-acid Esterase activity.

Plate Screen

Materials

• Ethyl ferulate solution (100mg/ml in dimethylformamide).
• Agar plates of media appropriate to your microorganism.
  • If screening natural strains some find it helpful to eliminate glucose from the media to drive FAE secretion.
  • This means that you will have to make this media yourself and can't buy a premix.
• Water
• Agar

Method

1. Grow colonies on agar plates of appropriate media until colonies reach a decent size. 2. For each plate to be screened, add 25-30mg agar to 6ml of water (i.e. if your screening 3 plates thats 80mg agar to 18ml water). 3. Microwave the agar mix until the agar is melted and put in 60°C water bath. 4. Once the media has been in the water bath for 15-20 mins:

1. Add the 30μL of ethyl ferulate solution (for every ml of top agar) and swirl to disperse (NO BUBBLES!).

• The ethyl ferulate will not really dissolve in the agar but should look cloudy.

2. Pour onto grown colonies immediately.

5. Incubate for ~4 hours 6. If a clear halo forms around the colony in the top agar then it's positive for FAE!!!

Assay

Materials

• Protein desalting columns
• HEPES
• sodium azide
• Dnase
• 4-nitrophenyl ferulic acid

Method

1. Make Protein buffer
   1. 100mM hepes
   2. 10μg/mL sodium Azide
   3. 5μL/mL Dnase
2. Concentrate cellular proteins from 1mL culture into 100μL buffer
3. Make Substrate buffer
   1. 2.5mM 4-nitrophenyl ferulic acid
   2. 0.5MKPO4
4. Add 20μL protein to 80μL substrate
5. Incubate for 30 mins at 37°C

Notes

• For the screen Donaghy et al. (1998) added the ethyl ferulate solution directly to the media plates at a concentration of 2mg/ml while Hassan and Pattat (2011) added it to the top agar at a stated concentration of 0.05mg/ml. We've found that the hassan and pattat concentration is too low to make the agar cloudy. But 0.5mg/ml works fine. -- Mike

References

Donaghy, J., P. F. Kelly, et al. (1998). "Detection of ferulic acid esterase production by Bacillus spp. and lactobacilli." Applied Microbiology and Biotechnology 50(2): 257-260.

Mastihuba, V., L. Kremnicky, et al. (2002). "A spectrophotometric assay for feruloyl esterases." Analytical Biochemistry 309(1): 96-101.

Nsereko, V. L., B. K. Smiley, et al. (2008). "Influence of inoculating forage with lactic acid bacterial strains that produce ferulate esterase on ensilage and ruminal degradation of fiber." Animal Feed Science and Technology 145(1-4): 122-135.

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