Ficoll - Revision history

Jakob Suckale: categories

Jakob Suckale — Thu, 21 Jan 2010 14:41:40 GMT

Jakob Suckale: see also

Jakob Suckale — Thu, 21 Jan 2010 14:40:57 GMT

see also

←Older revision		Revision as of 14:40, 21 January 2010
Line 1:		Line 1:
	'''Ficoll'''™ is a sucrose polymer commonly used to adjust the density and viscosity of solutions in the lab. It is employed in density gradients and loading buffers.		'''Ficoll'''™ is a sucrose polymer commonly used to adjust the density and viscosity of solutions in the lab. It is employed in density gradients and loading buffers.
		+
		+	== Use ==
		+	One of the most common applications for Ficoll is as a density gradient for the isolation of eukaryotic cells, organelles, bacterial cells, and pancreatic islets.

	== Properties ==		== Properties ==
	Ficoll is an uncharged, highly branched polymer formed by the co-polymerisation of sucrose and epichlorohydrin. Due to its many hydroxyl groups, Ficoll is highly soluble in water. Densities of up to 1.2 g/ml can be attained.		Ficoll is an uncharged, highly branched polymer formed by the co-polymerisation of sucrose and epichlorohydrin. Due to its many hydroxyl groups, Ficoll is highly soluble in water. Densities of up to 1.2 g/ml can be attained.

-	== ~~Use~~ ==	+	== Stability & storage ==
-	~~One of the most common applications for~~ Ficoll ~~is as a density gradient for~~ the ~~isolation of eukaryotic cells~~, ~~organelles~~, ~~bacterial cells~~, and ~~pancreatic islets~~.	+	Since Ficoll does not contain any ionized groups, the molecule does not readily react under physiological conditions. It is stable in alkaline and neutral solutions, but is rapidly hydrolysed in solution at pH 3,
		+	especially at high temperature. However, Ficoll can be autoclaving at 110ºC for 30 minutes in neutral solutions.
		+	Strong oxidizing and reducing agents should be avoided. Shipping and storage are at ambient temperatures.

	== See also ==		== See also ==
	* [http://en.wikipedia.org/wiki/Ficoll Ficoll at the Wikipedia]		* [http://en.wikipedia.org/wiki/Ficoll Ficoll at the Wikipedia]
		+	* [http://www.med.upenn.edu/bmcrc/immune/Ficcoll_info.pdf Ficoll 70 and 400 datasheet from Amersham at UPenn]
		+	* [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/f4375pis.Par.0001.File.tmp/f4375pis.pdf Ficoll 400 datasheet at Sigma-Aldrich]
		+	* [http://www4.gelifesciences.com/aptrix/upp01077.nsf/Content/cellpreparation_home~products_cell~density_gradient_media~ficoll_pm400?OpenDocument&moduleid=167163 Ficoll 400 page at GE Healthcare]

Jakob Suckale: properties & use

Jakob Suckale — Thu, 21 Jan 2010 14:35:18 GMT

properties & use

←Older revision		Revision as of 14:35, 21 January 2010
Line 1:		Line 1:
-	'''Ficoll'''~~<sup>TM</sup>~~ is ~~an uncharged, highly branched~~ polymer ~~formed by~~ the ~~co-polymerisation~~ of ~~sucrose~~ and ~~epichlorohydrin~~.	+	'''Ficoll'''™ is a sucrose polymer commonly used to adjust the density and viscosity of solutions in the lab. It is employed in density gradients and loading buffers.

-	http://en.wikipedia.org/wiki/Ficoll	+	== Properties ==
		+	Ficoll is an uncharged, highly branched polymer formed by the co-polymerisation of sucrose and epichlorohydrin. Due to its many hydroxyl groups, Ficoll is highly soluble in water. Densities of up to 1.2 g/ml can be attained.
		+
		+	== Use ==
		+	One of the most common applications for Ficoll is as a density gradient for the isolation of eukaryotic cells, organelles, bacterial cells, and pancreatic islets.
		+
		+	== See also ==
		+	* [http://en.wikipedia.org/wiki/Ficoll Ficoll at the Wikipedia]

Jakob Suckale: * page created *

Jakob Suckale — Thu, 21 Jan 2010 14:22:30 GMT

*** page created ***

New page

'''Ficoll'''<sup>TM</sup> is an uncharged, highly branched polymer formed by the co-polymerisation of sucrose and epichlorohydrin.

http://en.wikipedia.org/wiki/Ficoll

←Older revision		Revision as of 14:41, 21 January 2010
Line 17:		Line 17:
	* [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/f4375pis.Par.0001.File.tmp/f4375pis.pdf Ficoll 400 datasheet at Sigma-Aldrich]		* [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/f4375pis.Par.0001.File.tmp/f4375pis.pdf Ficoll 400 datasheet at Sigma-Aldrich]
	* [http://www4.gelifesciences.com/aptrix/upp01077.nsf/Content/cellpreparation_home~products_cell~density_gradient_media~ficoll_pm400?OpenDocument&moduleid=167163 Ficoll 400 page at GE Healthcare]		* [http://www4.gelifesciences.com/aptrix/upp01077.nsf/Content/cellpreparation_home~products_cell~density_gradient_media~ficoll_pm400?OpenDocument&moduleid=167163 Ficoll 400 page at GE Healthcare]
		+
		+
		+	[[Category:Material]]
		+	[[Category:Chemical]]

Ficoll - Revision history

Jakob Suckale: categories

Jakob Suckale: see also

Jakob Suckale: properties & use

Jakob Suckale: *** page created ***

Jakob Suckale: * page created *