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# Bell, Jason C., Plank, Jody L., Dombrowski, Christopher C., and Kowalczykowski. Direct imaging of RecA nucleation and growth on single molecules of SSB-coated ssDNA. Nature 491, 274 - 278 (2012). | # Bell, Jason C., Plank, Jody L., Dombrowski, Christopher C., and Kowalczykowski. Direct imaging of RecA nucleation and growth on single molecules of SSB-coated ssDNA. Nature 491, 274 - 278 (2012). | ||
# Chen, Z., Yang, H., Pavletich, N.P. Machanism of homolgous recombination from the RecA-ssDNA/dsDNA strucures. Nature 435. 489-494 (2008) | # Chen, Z., Yang, H., Pavletich, N.P. Machanism of homolgous recombination from the RecA-ssDNA/dsDNA strucures. Nature 435. 489-494 (2008) | ||
#Khanna, K. K. & Jackson, S. P. DNA double-strand breaks: signaling, repair and the cancer connection. Nature Genetics 27, 247 - 254 (2001) | # Khanna, K. K. & Jackson, S. P. DNA double-strand breaks: signaling, repair and the cancer connection. Nature Genetics 27, 247 - 254 (2001) | ||
# Meyer, R. R. & Laine, P. S. The single-stranded DNA-binding protein of Escherichia coli. Microbiology Review 54, 342-380 (1990) | |||
Revision as of 12:49, 5 February 2013
“Direct imaging of RecA nucleation and growth on single molecules of SSB-coated ssDNA” Jason C. Bell, Jody L. Plank, Christopher C. Dombrowski, and Stephen C. Kowalczykowski
Background
To ensure their survival, cells have evolved mechanisms by which they both monitor genome integrity and repair damage. The most dangerous type of DNA damage is double stranded breaks (DSB) which can be caused by endogenously generated oxygen radicals, replication forks encountering single stranded DNA breaks or other DNA lesions, or exogenous agents such as ionizing radiation or chemotherapeutic drugs [3].
Introduction
There are two main options for repairing a DSB: homologous recombination (HR) and non-homologous end joining (NHEJ) [3]. The focus of this paper is proteins, specifically RecA and SSB, involved in homologous recombination in E. coli.
RecA
RecA is a 38kDA ATPase, a class of enzymes that catalyze the hydrolysis of ATP, found in Escherichia coli that is essential for DNA repair and maintenance (1).
Single Strand DNA Binding Protein
Methods
Results
References
- Bell, Jason C., Plank, Jody L., Dombrowski, Christopher C., and Kowalczykowski. Direct imaging of RecA nucleation and growth on single molecules of SSB-coated ssDNA. Nature 491, 274 - 278 (2012).
- Chen, Z., Yang, H., Pavletich, N.P. Machanism of homolgous recombination from the RecA-ssDNA/dsDNA strucures. Nature 435. 489-494 (2008)
- Khanna, K. K. & Jackson, S. P. DNA double-strand breaks: signaling, repair and the cancer connection. Nature Genetics 27, 247 - 254 (2001)
- Meyer, R. R. & Laine, P. S. The single-stranded DNA-binding protein of Escherichia coli. Microbiology Review 54, 342-380 (1990)
