Fixing cells
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| - | '''Formaldehyde Fixation''' | + | |
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***This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy | ***This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy | ||
Revision as of 02:11, 26 February 2006
Formaldehyde Fixation
- This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy
1) Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature.
2) For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature. There is no need to wash between fixation and permeabilization steps.
