Fixing cells: Difference between revisions

Revision as of 23:12, 25 February 2006

- - This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy

Reagents -Fixation Solution: 4% Formaldehyde in PBS -Permeabilization Solution: 0.1% Triton X-100 in PBS

Protocol

1) For fixation, incubate cells in Formaldehyde Solution for 10-15 minutes at room temperature.

2) For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature.

@@ Line 1: / Line 1: @@
 == '''Formaldehyde Fixation''' ==
 ***This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy
-)  Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature.
+'''Reagents'''
+-Fixation Solution: 4% Formaldehyde in PBS
+-Permeabilization Solution: 0.1% Triton X-100 in PBS
+'''Protocol'''
+)  For fixation, incubate cells in Formaldehyde Solution for 10-15 minutes at room temperature.
-)  For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature.  There is no need to wash between fixation and permeabilization steps.
+)  For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature.