- This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy
Reagents -Fixation Solution: 4% Formaldehyde in PBS
-Permeabilization Solution: 0.1% Triton X-100 in PBS
1) For fixation, incubate cells in Formaldehyde Solution for 10-15 minutes at room temperature.
2) For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature.