Fluorescein diacetate: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(No difference)
|
Latest revision as of 10:12, 28 April 2005
- fluorescein (494/518) is formed by intracellular hydrolysis of FDA
- bacteria can hydrolyze this
- leaks from cells
- fluorescein has a relatively high rate of photobleaching
- pH-sensitive fluorescence ref (pKa ~6.4) that is significantly reduced below pH 7
- relatively broad fluorescence emission spectrum (not good for multicolor use)
