Fong:Protocols: Difference between revisions
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==Media and Reagents== | ==Media and Reagents== | ||
LB | *[[Fong:LB]] | ||
*[[Fong:Antibiotics]] - Antibiotic stock preparation | |||
[[Fong:Antibiotics]] - Antibiotic stock preparation | *CTFUD - Rich medium for ''C. thermocellum.'' Contact [[User:Christopher M Gowen|Chris]] for this protocol. | ||
*[[Fong:MJ]] - Minimal medium for ''C. thermocellum'' | |||
CTFUD - Rich medium for ''C. thermocellum.'' Contact [[User:Christopher M Gowen|Chris]] for this protocol. | *[[Fong:TFU]] - Medium for ''T. fusca'' | ||
[[Fong:MJ]] - Minimal medium for ''C. thermocellum'' | |||
[[Fong:TFU]] - Medium for ''T. fusca'' | |||
==General techniques== | ==General techniques== | ||
[[Fong:Lab Basics]] - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues | *[[Fong:Lab Basics]] - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues | ||
*[[Fong:Spectrophotometry]] and plate reader use | |||
[[Fong:Spectrophotometry]] and plate reader use | *[[Fong:Cryogenic storage]] | ||
*[[Fong:Serum bottle anaerobic culture]] - General methodology for sterile anaerobic bacterial culture | |||
[[Fong:Cryogenic storage]] | *[[Fong:Bradford protein assay]] - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates | ||
*[[Fong:Electrocompetency]] | |||
[[Fong:Serum bottle anaerobic culture]] - General methodology for sterile anaerobic bacterial culture | *[[Fong:Electrotransformation]] of ''E. coli'' | ||
*[[Fong:Transformation]] of ''T. fusca'' | |||
[[Fong:Bradford protein assay]] - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates | |||
[[Fong:Electrocompetency]] | |||
[[Fong:Electrotransformation]] of ''E. coli'' | |||
[[Fong:Transformation]] of ''T. fusca'' | |||
==Nucleic acids== | ==Nucleic acids== | ||
*[[Fong:RNA gel electrophoresis/Denaturing]] | |||
[[Fong:RNA gel electrophoresis/Denaturing]] | *[[Ethanol precipitation of nucleic acids]] - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435. | ||
*[[Fong:DNA gel electrophoresis]] | |||
[[Ethanol precipitation of nucleic acids]] - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435. | |||
[[Fong:DNA gel electrophoresis]] | |||
Revision as of 14:00, 3 June 2009
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Media and Reagents
- Fong:LB
- Fong:Antibiotics - Antibiotic stock preparation
- CTFUD - Rich medium for C. thermocellum. Contact Chris for this protocol.
- Fong:MJ - Minimal medium for C. thermocellum
- Fong:TFU - Medium for T. fusca
General techniques
- Fong:Lab Basics - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues
- Fong:Spectrophotometry and plate reader use
- Fong:Cryogenic storage
- Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture
- Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates
- Fong:Electrocompetency
- Fong:Electrotransformation of E. coli
- Fong:Transformation of T. fusca
Nucleic acids
- Fong:RNA gel electrophoresis/Denaturing
- Ethanol precipitation of nucleic acids - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435.
- Fong:DNA gel electrophoresis
