Fong:Protocols

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Media and Reagents

• Fong:LB
• Fong:Antibiotics - Antibiotic stock preparation
• Fong:IPTG - IPTG stock
• CTFUD - Rich medium for C. thermocellum. Contact Chris for this protocol.
• Fong:MJ - Minimal medium for C. thermocellum
• Fong:TFU - Medium for T. fusca

General techniques

• Fong:Lab Basics - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues
• Fong:Spectrophotometry and plate reader use
• Fong:Cryogenic storage
• Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture
• Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates
• Fong:Electrocompetency
• Fong:Electrotransformation of E. coli
• Fong:Transformation of T. fusca
• Fong:PCR
• Fong:Quantification of free Cellobiose

Nucleic acids

• Fong:Plasmid preparation
• Fong:RNA gel electrophoresis/Denaturing
• Ethanol precipitation of nucleic acids - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435.
• Fong:DNA gel electrophoresis
• Fong:BioBrick assembly