Fong:Protocols: Difference between revisions

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=Fong Lab Protocols=
=Fong Lab Protocols=


==Media and Reagents==
[[Fong:CTFUD]] - Rich medium for ''C. thermocellum''
[[Fong:MJ]] - Minimal medium for ''C. thermocellum''
==General techniques==
[[Fong:Serum bottle anaerobic culture]] - General methodology for sterile anaerobic bacterial culture
[[Fong:Serum bottle anaerobic culture]] - General methodology for sterile anaerobic bacterial culture


[[Fong:Bradford protein assay]] - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates
[[Fong:Bradford protein assay]] - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates


==Nucleic acids==
[[Fong:RNA gel electrophoresis/Denaturing]]
[[Fong:RNA gel electrophoresis/Denaturing]]

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Fong Lab Protocols

Media and Reagents

Fong:CTFUD - Rich medium for C. thermocellum

Fong:MJ - Minimal medium for C. thermocellum

General techniques

Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture

Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates

Nucleic acids

Fong:RNA gel electrophoresis/Denaturing