Fong:Protocols: Difference between revisions

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==Nucleic acids==
==Nucleic acids==
[[Fong:RNA gel electrophoresis/Denaturing]]
[[Fong:RNA gel electrophoresis/Denaturing]]
[[Fong:DNA precipitation]]
[[Fong:DNA gel electrophoresis]]

Revision as of 13:01, 16 April 2009

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Media and Reagents

Fong:CTFUD - Rich medium for C. thermocellum

Fong:MJ - Minimal medium for C. thermocellum

General techniques

Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture

Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates

Nucleic acids

Fong:RNA gel electrophoresis/Denaturing

Fong:DNA precipitation

Fong:DNA gel electrophoresis