Fong:Protocols: Difference between revisions
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[[Fong:Lab Basics]] - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues | [[Fong:Lab Basics]] - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues | ||
Spectrophotometry | [[Fong:Spectrophotometry]] and plate reader use | ||
[[Fong:Cryogenic storage]] | [[Fong:Cryogenic storage]] | ||
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[[Fong:Bradford protein assay]] - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates | [[Fong:Bradford protein assay]] - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates | ||
[[Fong:Electrotransformation]] of ''E. coli'' | |||
==Nucleic acids== | ==Nucleic acids== | ||
Revision as of 13:37, 1 June 2009
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Media and Reagents
Fong:CTFUD - Rich medium for C. thermocellum
Fong:MJ - Minimal medium for C. thermocellum
Fong:TFU - Medium for T. fusca
LB broth / plates
General techniques
Fong:Lab Basics - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues
Fong:Spectrophotometry and plate reader use
Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture
Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates
Fong:Electrotransformation of E. coli
Nucleic acids
Fong:RNA gel electrophoresis/Denaturing
Ethanol precipitation of nucleic acids - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435.
