Fong:Protocols

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(General techniques)
(Media and Reagents)
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==Media and Reagents==
==Media and Reagents==
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[[Fong:CTFUD]] - Rich medium for ''C. thermocellum''
+
LB broth / plates
 +
 
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CTFUD - Rich medium for ''C. thermocellum.'' Contact [[User:Christopher M Gowen|Chris]] for this protocol.
[[Fong:MJ]] - Minimal medium for ''C. thermocellum''
[[Fong:MJ]] - Minimal medium for ''C. thermocellum''
[[Fong:TFU]] - Medium for ''T. fusca''
[[Fong:TFU]] - Medium for ''T. fusca''
-
 
-
LB broth / plates
 
==General techniques==
==General techniques==

Revision as of 21:56, 1 June 2009

Cth.JPG


Media and Reagents

LB broth / plates

CTFUD - Rich medium for C. thermocellum. Contact Chris for this protocol.

Fong:MJ - Minimal medium for C. thermocellum

Fong:TFU - Medium for T. fusca

General techniques

Fong:Lab Basics - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues

Fong:Spectrophotometry and plate reader use

Fong:Cryogenic storage

Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture

Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates

Fong:Electrotransformation of E. coli

Fong:Transformation of T. fusca

Nucleic acids

Fong:RNA gel electrophoresis/Denaturing

Ethanol precipitation of nucleic acids - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435.

Fong:DNA gel electrophoresis
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