Fong:Protocols
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==Media and Reagents== | ==Media and Reagents== | ||
| - | + | LB broth / plates | |
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| + | CTFUD - Rich medium for ''C. thermocellum.'' Contact [[User:Christopher M Gowen|Chris]] for this protocol. | ||
[[Fong:MJ]] - Minimal medium for ''C. thermocellum'' | [[Fong:MJ]] - Minimal medium for ''C. thermocellum'' | ||
[[Fong:TFU]] - Medium for ''T. fusca'' | [[Fong:TFU]] - Medium for ''T. fusca'' | ||
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==General techniques== | ==General techniques== | ||
Revision as of 22:56, 1 June 2009
Media and Reagents
LB broth / plates
CTFUD - Rich medium for C. thermocellum. Contact Chris for this protocol.
Fong:MJ - Minimal medium for C. thermocellum
Fong:TFU - Medium for T. fusca
General techniques
Fong:Lab Basics - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues
Fong:Spectrophotometry and plate reader use
Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture
Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates
Fong:Electrotransformation of E. coli
Fong:Transformation of T. fusca
Nucleic acids
Fong:RNA gel electrophoresis/Denaturing
Ethanol precipitation of nucleic acids - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435.
Fong:DNA gel electrophoresis
