Fong:TFU

Overview

This liquid medium is for general culture of the actinomycete Thermobifida fusca. In this recipe, cellobiose is the sole carbon source at 0.5%. Both the amount and type of carbon source may be varied; cellulose, CMC, glucose, and xylan are all acceptable sources.

Materials

For one liter liquid medium

• Macro Salts
  • 1.5g NaCl
  • 3.1g (NH)₂SO₄
  • 9.1g Na₂HPO₄
  • 0.9g KH₂HPO₄
• 0.2/1g Yeast Extract
• 5.0g Cellobiose
• 20ml [Microsalts]
• 10ml Biotin and Thiamine

Procedure

1. Add macrosalts to 750ml diH₂O
   • Heat as necessary.
2. Adjust to 7.4 ± 0.2 pH
3. Add additional ingredients
4. Raise volume to 1L and cover
5. Autoclave on liquid cycle for at least 30min.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

1. Remember that T. fusca is a thermophile; optimal T_growth is 55°C

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

Contact

• Deng

or instead, discuss this protocol.