Frankel:Force Spectroscopy
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| + | '''<font align="center" font color=#ffffff font size=8>______ </font>''''''<font align="center" font color=#000000 font size=8>Force Spectroscopy</font>''' | ||
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| + | | align="center" width=400px style="border: 2px solid #000033; background-color:#FFFFFF; padding:1em;" class="plainlinks" valign="top"| | ||
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| + | '''<font color=#000000 font size=3>Picking up and unfolding a single protein</font>''' | ||
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| + | [[Image:Spectroscopy_complete.jpg|350px]] | ||
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| + | | align="center" rowspan="2" width=21px style="border: 2px solid #000033; background-color:#ffffff; padding:1em;" valign="top"| | ||
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| + | '''<font color=#FFFFFF font size=3>Assembly</font>''' | ||
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| + | '''<font color=#000000 font size=3>Nano-mechanics of HIV GP160</font>''' | ||
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| + | [[Image:GP160mica.png|300px]] | ||
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| + | [[Image:GP_160.png|300px]] | ||
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| + | '''<font color=#FFFFFF font size=6>GP160mica</font>''' | ||
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| + | [[Image:Stlow.png|330px]] | ||
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| + | [[Image:Hislow.png|330px]] | ||
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| + | ---- | ||
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| + | '''<font color=#000000 font size=3 font align="justify"> | ||
| + | Typical sawtooth unfolding pattern of a single molecule of the HIV virion protein GP160. Unfolding forces can be extracted from the sawtooth pattern by applying the worm like chain model.</font>''' | ||
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| + | {| style="width: 728px; text-align:center;font-size:12px;font-variant: small-caps;width: 18px; " align="justify" | ||
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| + | <br /> | ||
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| + | |---- | ||
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| + | | align="center" width=21px style="border: 2px solid #000000; background-color:#FFFFFF; padding:1em;" valign="top"| | ||
| + | '''<font color=#000000 font size=3> Unfolding the ECM protein fibronectin </font>''' | ||
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| + | [[Image:FNsawt.png|300px]] | ||
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| + | [[Image:HisFN.png|300px]] | ||
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| + | ---- | ||
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| + | '''<font align="justify" font size=3> | ||
| + | Sawtooth pattern on the retraction force curve indicating the unfolding of fibronectin. The average rupture force distribution of the protein on mica surface was 85.1 ± 2.7 pN. | ||
Current revision
'______ 'Force Spectroscopy
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Picking up and unfolding a single protein
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Assembly Nano-mechanics of HIV GP160
GP160mica
Typical sawtooth unfolding pattern of a single molecule of the HIV virion protein GP160. Unfolding forces can be extracted from the sawtooth pattern by applying the worm like chain model.
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Unfolding the ECM protein fibronectin
Sawtooth pattern on the retraction force curve indicating the unfolding of fibronectin. The average rupture force distribution of the protein on mica surface was 85.1 ± 2.7 pN. |
