Frankel:Force Spectroscopy

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (20:22, 9 January 2013) (view source)
 
(3 intermediate revisions not shown.)
Line 9: Line 9:
<div>
<div>
-
|  align="justify" width=400px style="border: 2px solid #000033; background-color:#FFFFFF; padding:1em;" class="plainlinks" valign="top"|
+
|  align="center" width=400px style="border: 2px solid #000033; background-color:#FFFFFF; padding:1em;" class="plainlinks" valign="top"|
-
'''<font color=#FFFFFF font size=8>HIV-gp160</font>'''
+
 
 +
 
 +
'''<font color=#000000 font size=3>Picking up and unfolding a single protein</font>'''
[[Image:Spectroscopy_complete.jpg|350px]]
[[Image:Spectroscopy_complete.jpg|350px]]
Line 25: Line 27:
[[Image:GP160mica.png|300px]]
[[Image:GP160mica.png|300px]]
 +
 +
 +
[[Image:GP_160.png|300px]]
'''<font color=#FFFFFF font size=6>GP160mica</font>'''
'''<font color=#FFFFFF font size=6>GP160mica</font>'''
[[Image:Stlow.png|330px]]
[[Image:Stlow.png|330px]]
 +
[[Image:Hislow.png|330px]]
[[Image:Hislow.png|330px]]
 +
----
----
Line 53: Line 60:
| align="center" width=21px style="border: 2px solid #000000; background-color:#FFFFFF; padding:1em;" valign="top"|
| align="center" width=21px style="border: 2px solid #000000; background-color:#FFFFFF; padding:1em;" valign="top"|
'''<font color=#000000 font size=3> Unfolding the ECM protein fibronectin </font>'''
'''<font color=#000000 font size=3> Unfolding the ECM protein fibronectin </font>'''
-
 
[[Image:FNsawt.png|300px]]
[[Image:FNsawt.png|300px]]
-
 
[[Image:HisFN.png|300px]]
[[Image:HisFN.png|300px]]

Current revision

Danbanner-bio-machines.jpg



'______ 'Force Spectroscopy


Assembly

Nano-mechanics of HIV GP160



GP160mica



Typical sawtooth unfolding pattern of a single molecule of the HIV virion protein GP160. Unfolding forces can be extracted from the sawtooth pattern by applying the worm like chain model.





Unfolding the ECM protein fibronectin



Sawtooth pattern on the retraction force curve indicating the unfolding of fibronectin. The average rupture force distribution of the protein on mica surface was 85.1 ± 2.7 pN.

Personal tools