Freimoser:Recipes:TransferBuffer: Difference between revisions
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==Transfer Buffer== | ==Transfer Buffer== | ||
Revision as of 08:04, 2 November 2006
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Transfer Buffer
Final working concentration (1x)
- 3 g Tris
- 14.4 g Glycine
- 800 mL H2O
- 200 mL Methanol
10x stock solution
- 30 g Tris
- 144 g Glycine
Fill up to 800 mL with H2O.
To make the 1x working solution from this stock use:
- 80 mL 10x stock solution
- 720 mL H2O
- 200 mL Methanol
