Freimoser:Recipes:Yeast:SC: Difference between revisions
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SC medium contains yeast nitrogen base, ammonium sulfate, a carbon source (usually glucose) and amino acids. Depending on the specific strains and experiments different variations are possible. | SC medium contains yeast nitrogen base, ammonium sulfate, a carbon source (usually glucose) and amino acids. Depending on the specific strains and experiments different variations are possible. | ||
==Recipe for 1 liter== | ===Recipe for 1 liter=== | ||
#6.8 g Yeast nitrogen base (YNB, including ammonium sulfate, from [http://www.formedium.com/ Formedium]) | #6.8 g Yeast nitrogen base (YNB, including ammonium sulfate, from [http://www.formedium.com/ Formedium]) | ||
| Line 16: | Line 16: | ||
==Modifications== | ===Modifications=== | ||
#For SC-URA use 0.77 g/L CSM–URA drop-out mix [http://www.qbiogene.com/ Qbiogene] | #For SC-URA use 0.77 g/L CSM–URA drop-out mix [http://www.qbiogene.com/ Qbiogene] | ||
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#To test nitrogen sources Yeast Carbon Base (YCB) can be used (from [http://www.formedium.com/ Formedium]). YCB already contains 10.15 g glucose! Therefore, add only 10 g glucose for 1 L of medium. | #To test nitrogen sources Yeast Carbon Base (YCB) can be used (from [http://www.formedium.com/ Formedium]). YCB already contains 10.15 g glucose! Therefore, add only 10 g glucose for 1 L of medium. | ||
==Remarks== | ===Remarks=== | ||
#many different drop-out amino acid mixtures are available either from [http://www.qbiogene.com/ Qbiogene] or [http://www.formedium.com/ Formedium] | #many different drop-out amino acid mixtures are available either from [http://www.qbiogene.com/ Qbiogene] or [http://www.formedium.com/ Formedium] | ||
#different versions of YNB are available from the same suppliers | #different versions of YNB are available from the same suppliers | ||
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SC medium
SC medium contains yeast nitrogen base, ammonium sulfate, a carbon source (usually glucose) and amino acids. Depending on the specific strains and experiments different variations are possible.
Recipe for 1 liter
- 6.8 g Yeast nitrogen base (YNB, including ammonium sulfate, from Formedium)
- 0.79 g Complete supplement mixture (CSM, from Formedium)
- Fill up to 950 ml and autoclave
- Add 50 ml of a 40% glucose solution that was autoclaved separately
- If solid medium is required, add 20 g/L agar prior to autoclaving
Modifications
- For SC-URA use 0.77 g/L CSM–URA drop-out mix Qbiogene
- For SC-P use 0.7 g/L phosphate free YNB (Qbiogene). The desired amount of phosphate can then be added separately. We use YNB without ammonium sulfate and potassium phosphate. Therefore, ammonium sulfate must be added separately!
- To test nitrogen sources Yeast Carbon Base (YCB) can be used (from Formedium). YCB already contains 10.15 g glucose! Therefore, add only 10 g glucose for 1 L of medium.
