Freimoser:Research
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Research
The focus of the research in our group is a simple
molecule: inorganic
polyphosphate (poly P).
Poly P is a linear polymer that consists of a variable number of
phosphate residues
(from three to more than a thousand) that are
linked by energy-rich phosphoanhydride bonds. It has been detected
in all organisms and living cells and was found in many organelles.
In eukaryotes, poly P is particularly
prominent in fungi, algae and trypanosomatids.
Poly P serves as a phosphate and energy store and regulates
enzymes, chromatin condensation
and translation. Poly P is also involved in bacterial pathogenicity,
survival during stationary phase in
bacteria and yeast, or the adaptation to alkaline and osmotic stress.
In the slime mold Dictyostelium discoideum, poly P regulates
development and predation behaviour, and in humans blood coagulation is
accelerated and fibrinolysis is delayed by poly P.
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Poly P in Saccharomyces cerevisiae:We have developed a fast and easy method for the quantification of poly P in yeast, which is amenable for large-scale analyses. This allowed extraction and quantification of poly P in mutant strains of all non-essential yeast genes. From this screen we conclude that at least 5% of all yeast genes are required for the maintenance of normal poly P levels. We are now studying specific pathways to determine their link with poly P metabolism. In addition, we study phenotypical differences in poly P hypo- and hyper-accumulating strains in order to learn more about the biological significance of poly P accumulation in yeast. |
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Cell wall localized poly P:One of our major goals and a very difficult problem is the specific localization of poly P. We have developed a staining method that is based on poly P binding proteins and immunohistochemical detection that allows for highly sensitive localization of poly P in fungal cell walls. We have used this technique for a systematic study of poly P in fungi from all fungal phyla. In the future we will also define procedures to stain poly P intracellularly. |
