French Lab: Difference between revisions
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*Flavin-dependent monooxygenases: Peter Wilson | *Flavin-dependent monooxygenases: Peter Wilson | ||
*[[Cfrench:hemtoppage|Bacteriohemerythrins]]: C. French | *[[Cfrench:hemtoppage|Bacteriohemerythrins]]: C. French | ||
===Protocols=== | ===Biobrick Protocols=== | ||
*[[Cfrench:bbprimerdesign|1. Primer Design]] | |||
*[[Cfrench:PfuPCR|2. Cloning parts by PCR with Pfu polymerase]] | |||
*[[Cfrench:AGE|3. Agarose Gel Electrophoresis]] | |||
*[[Cfrench:DNAPurification1|4. Purifying a DNA product from solution]] | |||
*[[Cfrench:bbcloning|5. Cloning a PCR product into a biobrick vector]] | |||
*[[Cfrench:compcellprep1|6. Preparing and using competent E. coli cells]] | |||
*[[Cfrench:PCRScreening|7. Screening colonies by PCR]] | |||
*[[Cfrench:minipreps1|8. Plasmid DNA minipreps]] | |||
*[[Cfrench:restriction1|9. Analytical restriction digests]] | |||
*[[Cfrench:bbcombining1|10. Combining two biobricks to make a new biobrick]] | |||
*[[Cfrench:addingrbs|11. Adding a ribosome binding site to a biobrick coding sequence]] | |||
===Other Protocols=== | |||
*Growth of E. coli | *Growth of E. coli | ||
*Growth of B. subtilis | *Growth of B. subtilis | ||
Revision as of 07:58, 8 July 2007
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