French Lab
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===Projects=== | ===Projects=== | ||
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*Flavin-dependent monooxygenases: Peter Wilson | *Flavin-dependent monooxygenases: Peter Wilson | ||
*[[Cfrench:hemtoppage|Bacteriohemerythrins]]: C. French | *[[Cfrench:hemtoppage|Bacteriohemerythrins]]: C. French | ||
| - | ===Protocols=== | + | *Biofilms: Gary Dorken |
| + | *[[Cfrench:BioBrickVectors1|Making a broad host range BioBrick vector]]: Edinburgh iGEM2007 team and C. French | ||
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| + | ===Outreach=== | ||
| + | *[[Cfrench:ScienceFiction|1. Portrayal of Scientists in Science Fiction]] | ||
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| + | ===Workshops=== | ||
| + | *[[Cfrench:CaseStudy1|Synthetic Biology Case Study 1: development of an arsenic biosensor]] | ||
| + | *[[Cfrench:CaseStudy2|Synthetic Biology Case Study 2: development of processes for bioconversion of cellulosic materials]] | ||
| + | |||
| + | ===Biobrick Protocols=== | ||
| + | *[[Cfrench:bbprimerdesign|1. Primer Design]] | ||
| + | *[[Cfrench:PfuPCR|2. Cloning parts by PCR with Pfu polymerase]] | ||
| + | *[[Cfrench:KodPCR|2b. Cloning parts by PCR with Kod polymerase]] | ||
| + | *[[Cfrench:AGE|3. Agarose Gel Electrophoresis]] | ||
| + | *[[Cfrench:DNAPurification1|4. Purifying a PCR product from solution]] | ||
| + | *[[Cfrench:bbcloning|5. Cloning a PCR product into a biobrick vector]] | ||
| + | *[[Cfrench:compcellprep1|6. Preparing and using competent E. coli cells]] | ||
| + | *[[Cfrench:PCRScreening|7. Screening colonies by PCR]] | ||
| + | *[[Cfrench:minipreps1|8. Plasmid DNA minipreps]] | ||
| + | *[[Cfrench:maxipreps1|8b. Plasmid DNA maxipreps]] | ||
| + | *[[Cfrench:restriction1|9. Analytical restriction digests]] | ||
| + | *[[Cfrench:bbcombining1|10. Combining two biobricks to make a new biobrick]] | ||
| + | *[[Cfrench:addingrbs|11. Adding a ribosome binding site to a biobrick coding sequence]] | ||
| + | *[[Cfrench:BALTIC|12. BALTIC: a new method for combining standard BioBricks]] | ||
| + | *[[Cfrench:MABEL|13. MABEL: a fast easy method for removing unwanted restriction sites]] | ||
| + | *14. BABEL: a new method for generating BioBricks without restriction digests. | ||
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| + | ===Other Protocols=== | ||
*Growth of E. coli | *Growth of E. coli | ||
*Growth of B. subtilis | *Growth of B. subtilis | ||
| + | *[[Cfrench:BacTrans1|Transforming ''Bacillus subtilis'']] | ||
| + | *[[Cfrench:BacTrans2|Transforming ''Bacillus subtilis'' - 2]] | ||
*[[Cfrench:minipreps1|Plasmid DNA minipreps]] | *[[Cfrench:minipreps1|Plasmid DNA minipreps]] | ||
*Restriction digests | *Restriction digests | ||
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*Cloning of PCR products | *Cloning of PCR products | ||
*Preparation of cell extracts | *Preparation of cell extracts | ||
| - | *Protein Assay | + | *[[Cfrench:ProteinAssay|Protein Assay]] |
*[[Cfrench:sdspage1|SDS-PAGE]] | *[[Cfrench:sdspage1|SDS-PAGE]] | ||
*Native-PAGE | *Native-PAGE | ||
*Enrichment and growth of environmental bacteria | *Enrichment and growth of environmental bacteria | ||
| - | *Identification of bacteria | + | *[[Cfrench:IdentifyingBacteria|Identification of bacteria]] |
*[[Cfrench:gramstain|Gram stain]] | *[[Cfrench:gramstain|Gram stain]] | ||
| + | *[[Cfrench:glycerol|Making glycerol stocks]] | ||
| + | *[[Cfrench:anthrone|Assaying polysaccharides with the anthrone assay]] | ||
| + | *[[Cfrench:Sporulation|Production of ''B. subtilis'' endospores]] | ||
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| + | ===Lab information=== | ||
| + | [[Cfrench:primerlist|Catalog of primers]] | ||
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===Safety Information=== | ===Safety Information=== | ||
[[Cfrench:toxiclist|Hazardous chemicals list]] | [[Cfrench:toxiclist|Hazardous chemicals list]] | ||
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[http://www.biology.ed.ac.uk/people/homepage.php?id=cfrench SBS page] | [http://www.biology.ed.ac.uk/people/homepage.php?id=cfrench SBS page] | ||
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[http://www.ed.ac.uk/ University of Edinburgh.] | [http://www.ed.ac.uk/ University of Edinburgh.] | ||
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| + | __NOTOC__ | ||
Current revision
