French Lab

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(Other Protocols)
 
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*Flavin-dependent monooxygenases: Peter Wilson
*Flavin-dependent monooxygenases: Peter Wilson
*[[Cfrench:hemtoppage|Bacteriohemerythrins]]: C. French
*[[Cfrench:hemtoppage|Bacteriohemerythrins]]: C. French
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*Biofilms: Gary Dorken
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*[[Cfrench:BioBrickVectors1|Making a broad host range BioBrick vector]]: Edinburgh iGEM2007 team and C. French
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===Outreach===
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*[[Cfrench:ScienceFiction|1. Portrayal of Scientists in Science Fiction]]
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===Workshops===
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*[[Cfrench:CaseStudy1|Synthetic Biology Case Study 1: development of an arsenic biosensor]]
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*[[Cfrench:CaseStudy2|Synthetic Biology Case Study 2: development of processes for bioconversion of cellulosic materials]]
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===Biobrick Protocols===
===Biobrick Protocols===
*[[Cfrench:bbprimerdesign|1. Primer Design]]
*[[Cfrench:bbprimerdesign|1. Primer Design]]
*[[Cfrench:PfuPCR|2. Cloning parts by PCR with Pfu polymerase]]
*[[Cfrench:PfuPCR|2. Cloning parts by PCR with Pfu polymerase]]
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*[[Cfrench:KodPCR|2b. Cloning parts by PCR with Kod polymerase]]
*[[Cfrench:AGE|3. Agarose Gel Electrophoresis]]
*[[Cfrench:AGE|3. Agarose Gel Electrophoresis]]
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*[[Cfrench:DNAPurification1|4. Purifying a DNA product from solution]]
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*[[Cfrench:DNAPurification1|4. Purifying a PCR product from solution]]
*[[Cfrench:bbcloning|5. Cloning a PCR product into a biobrick vector]]
*[[Cfrench:bbcloning|5. Cloning a PCR product into a biobrick vector]]
*[[Cfrench:compcellprep1|6. Preparing and using competent E. coli cells]]
*[[Cfrench:compcellprep1|6. Preparing and using competent E. coli cells]]
*[[Cfrench:PCRScreening|7. Screening colonies by PCR]]
*[[Cfrench:PCRScreening|7. Screening colonies by PCR]]
*[[Cfrench:minipreps1|8. Plasmid DNA minipreps]]
*[[Cfrench:minipreps1|8. Plasmid DNA minipreps]]
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*[[Cfrench:maxipreps1|8b. Plasmid DNA maxipreps]]
*[[Cfrench:restriction1|9. Analytical restriction digests]]
*[[Cfrench:restriction1|9. Analytical restriction digests]]
*[[Cfrench:bbcombining1|10. Combining two biobricks to make a new biobrick]]
*[[Cfrench:bbcombining1|10. Combining two biobricks to make a new biobrick]]
*[[Cfrench:addingrbs|11. Adding a ribosome binding site to a biobrick coding sequence]]
*[[Cfrench:addingrbs|11. Adding a ribosome binding site to a biobrick coding sequence]]
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*[[Cfrench:BALTIC|12. BALTIC: a new method for combining standard BioBricks]]
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*[[Cfrench:MABEL|13. MABEL: a fast easy method for removing unwanted restriction sites]]
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*14. BABEL: a new method for generating BioBricks without restriction digests.
===Other Protocols===
===Other Protocols===
*Growth of E. coli
*Growth of E. coli
*Growth of B. subtilis
*Growth of B. subtilis
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*[[Cfrench:BacTrans1|Transforming ''Bacillus subtilis'']]
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*[[Cfrench:BacTrans2|Transforming ''Bacillus subtilis'' - 2]]
*[[Cfrench:minipreps1|Plasmid DNA minipreps]]
*[[Cfrench:minipreps1|Plasmid DNA minipreps]]
*Restriction digests
*Restriction digests
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*Cloning of PCR products
*Cloning of PCR products
*Preparation of cell extracts
*Preparation of cell extracts
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*Protein Assay
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*[[Cfrench:ProteinAssay|Protein Assay]]
*[[Cfrench:sdspage1|SDS-PAGE]]
*[[Cfrench:sdspage1|SDS-PAGE]]
*Native-PAGE
*Native-PAGE
*Enrichment and growth of environmental bacteria
*Enrichment and growth of environmental bacteria
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*Identification of bacteria
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*[[Cfrench:IdentifyingBacteria|Identification of bacteria]]
*[[Cfrench:gramstain|Gram stain]]
*[[Cfrench:gramstain|Gram stain]]
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*[[Cfrench:glycerol|Making glycerol stocks]]
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*[[Cfrench:anthrone|Assaying polysaccharides with the anthrone assay]]
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*[[Cfrench:Sporulation|Production of ''B. subtilis'' endospores]]
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===Lab information===
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[[Cfrench:primerlist|Catalog of primers]]
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===Safety Information===
===Safety Information===
[[Cfrench:toxiclist|Hazardous chemicals list]]
[[Cfrench:toxiclist|Hazardous chemicals list]]

Current revision

Lab banner

Version: 22 May 2007

Projects

  • Magnetic bacteria: Jen Bell
  • Magnetic bacteria: Dzianis Trubitsyn
  • Conventional whole cell biosensors: Chris French
  • 'Bacillosensors': Chris French and Lucy Montgomery
  • Synthetic biology-based biosensors: Chris French, Xiaonan Wang, Nimisha Joshi
  • Phytoremediation: Chris French
  • Thiocyanate degradation: Chris French
  • Flavin-dependent monooxygenases: Peter Wilson
  • Bacteriohemerythrins: C. French
  • Biofilms: Gary Dorken
  • Making a broad host range BioBrick vector: Edinburgh iGEM2007 team and C. French

Outreach

Workshops

Biobrick Protocols

Other Protocols

Lab information

Catalog of primers

Safety Information

Hazardous chemicals list

Other links

C. French main page

Bacteriology page

SBS page

University of Edinburgh.

Personal tools