French Lab: Difference between revisions
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*Flavin-dependent monooxygenases: Peter Wilson | *Flavin-dependent monooxygenases: Peter Wilson | ||
*[[Cfrench:hemtoppage|Bacteriohemerythrins]]: C. French | *[[Cfrench:hemtoppage|Bacteriohemerythrins]]: C. French | ||
*Biofilms: Gary Dorken | |||
*[[Cfrench:BioBrickVectors1|Making a broad host range BioBrick vector]]: Edinburgh iGEM2007 team and C. French | |||
===Outreach=== | |||
*[[Cfrench:ScienceFiction|1. Portrayal of Scientists in Science Fiction]] | |||
===Workshops=== | |||
*[[Cfrench:CaseStudy1|Synthetic Biology Case Study 1: development of an arsenic biosensor]] | |||
*[[Cfrench:CaseStudy2|Synthetic Biology Case Study 2: development of processes for bioconversion of cellulosic materials]] | |||
===Biobrick Protocols=== | ===Biobrick Protocols=== | ||
*[[Cfrench:bbprimerdesign|1. Primer Design]] | *[[Cfrench:bbprimerdesign|1. Primer Design]] | ||
*[[Cfrench:PfuPCR|2. Cloning parts by PCR with Pfu polymerase]] | *[[Cfrench:PfuPCR|2. Cloning parts by PCR with Pfu polymerase]] | ||
*[[Cfrench:KodPCR|2b. Cloning parts by PCR with Kod polymerase]] | |||
*[[Cfrench:AGE|3. Agarose Gel Electrophoresis]] | *[[Cfrench:AGE|3. Agarose Gel Electrophoresis]] | ||
*[[Cfrench:DNAPurification1|4. Purifying a | *[[Cfrench:DNAPurification1|4. Purifying a PCR product from solution]] | ||
*[[Cfrench:bbcloning|5. Cloning a PCR product into a biobrick vector]] | *[[Cfrench:bbcloning|5. Cloning a PCR product into a biobrick vector]] | ||
*[[Cfrench:compcellprep1|6. Preparing and using competent E. coli cells]] | *[[Cfrench:compcellprep1|6. Preparing and using competent E. coli cells]] | ||
*[[Cfrench:PCRScreening|7. Screening colonies by PCR]] | *[[Cfrench:PCRScreening|7. Screening colonies by PCR]] | ||
*[[Cfrench:minipreps1|8. Plasmid DNA minipreps]] | *[[Cfrench:minipreps1|8. Plasmid DNA minipreps]] | ||
*[[Cfrench:maxipreps1|8b. Plasmid DNA maxipreps]] | |||
*[[Cfrench:restriction1|9. Analytical restriction digests]] | *[[Cfrench:restriction1|9. Analytical restriction digests]] | ||
*[[Cfrench:bbcombining1|10. Combining two biobricks to make a new biobrick]] | *[[Cfrench:bbcombining1|10. Combining two biobricks to make a new biobrick]] | ||
*[[Cfrench:addingrbs|11. Adding a ribosome binding site to a biobrick coding sequence]] | *[[Cfrench:addingrbs|11. Adding a ribosome binding site to a biobrick coding sequence]] | ||
*[[Cfrench:BALTIC|12. BALTIC: a new method for combining standard BioBricks]] | |||
*[[Cfrench:MABEL|13. MABEL: a fast easy method for removing unwanted restriction sites]] | |||
*14. BABEL: a new method for generating BioBricks without restriction digests. | |||
===Other Protocols=== | ===Other Protocols=== | ||
*Growth of E. coli | *Growth of E. coli | ||
*Growth of B. subtilis | *Growth of B. subtilis | ||
*[[Cfrench:BacTrans1|Transforming ''Bacillus subtilis'']] | |||
*[[Cfrench:BacTrans2|Transforming ''Bacillus subtilis'' - 2]] | |||
*[[Cfrench:minipreps1|Plasmid DNA minipreps]] | *[[Cfrench:minipreps1|Plasmid DNA minipreps]] | ||
*Restriction digests | *Restriction digests | ||
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*Cloning of PCR products | *Cloning of PCR products | ||
*Preparation of cell extracts | *Preparation of cell extracts | ||
*Protein Assay | *[[Cfrench:ProteinAssay|Protein Assay]] | ||
*[[Cfrench:sdspage1|SDS-PAGE]] | *[[Cfrench:sdspage1|SDS-PAGE]] | ||
*Native-PAGE | *Native-PAGE | ||
*Enrichment and growth of environmental bacteria | *Enrichment and growth of environmental bacteria | ||
*Identification of bacteria | *[[Cfrench:IdentifyingBacteria|Identification of bacteria]] | ||
*[[Cfrench:gramstain|Gram stain]] | *[[Cfrench:gramstain|Gram stain]] | ||
*[[Cfrench:glycerol|Making glycerol stocks]] | |||
*[[Cfrench:anthrone|Assaying polysaccharides with the anthrone assay]] | |||
*[[Cfrench:Sporulation|Production of ''B. subtilis'' endospores]] | |||
===Lab information=== | |||
[[Cfrench:primerlist|Catalog of primers]] | |||
===Safety Information=== | ===Safety Information=== | ||
[[Cfrench:toxiclist|Hazardous chemicals list]] | [[Cfrench:toxiclist|Hazardous chemicals list]] | ||
Revision as of 09:41, 14 November 2012
