French Lab

Version: 22 May 2007

Projects

• Magnetic bacteria: Jen Bell
• Magnetic bacteria: Dzianis Trubitsyn
• Conventional whole cell biosensors: Chris French
• 'Bacillosensors': Chris French and Lucy Montgomery
• Synthetic biology-based biosensors: Chris French, Xiaonan Wang, Nimisha Joshi
• Phytoremediation: Chris French
• Thiocyanate degradation: Chris French
• Flavin-dependent monooxygenases: Peter Wilson
• Bacteriohemerythrins: C. French
• Biofilms: Gary Dorken
• Making a broad host range BioBrick vector: Edinburgh iGEM2007 team and C. French

Outreach

• 1. Portrayal of Scientists in Science Fiction

Workshops

• Synthetic Biology Case Study 1: development of an arsenic biosensor
• Synthetic Biology Case Study 2: development of processes for bioconversion of cellulosic materials

Biobrick Protocols

• 1. Primer Design
• 2. Cloning parts by PCR with Pfu polymerase
• 2b. Cloning parts by PCR with Kod polymerase
• 3. Agarose Gel Electrophoresis
• 4. Purifying a PCR product from solution
• 5. Cloning a PCR product into a biobrick vector
• 6. Preparing and using competent E. coli cells
• 7. Screening colonies by PCR
• 8. Plasmid DNA minipreps
• 8b. Plasmid DNA maxipreps
• 9. Analytical restriction digests
• 10. Combining two biobricks to make a new biobrick
• 11. Adding a ribosome binding site to a biobrick coding sequence
• 12. BALTIC: a new method for combining standard BioBricks
• 13. MABEL: a fast easy method for removing unwanted restriction sites
• 14. BABEL: a new method for generating BioBricks without restriction digests.

Other Protocols

• Growth of E. coli
• Growth of B. subtilis
• Transforming Bacillus subtilis
• Transforming Bacillus subtilis - 2
• Plasmid DNA minipreps
• Restriction digests
• Agarose gel electrophoresis
• PCR
• Purification of DNA fragments
• Ligation
• Cloning of PCR products
• Preparation of cell extracts
• Protein Assay
• SDS-PAGE
• Native-PAGE
• Enrichment and growth of environmental bacteria
• Identification of bacteria
• Gram stain
• Making glycerol stocks
• Assaying polysaccharides with the anthrone assay
• Production of B. subtilis endospores

Lab information

Catalog of primers

Safety Information

Hazardous chemicals list

Other links

C. French main page

Bacteriology page

SBS page

University of Edinburgh.