G.tigrina Hox gene DthoxC insertion into prokaryote E.coli / (by UNIamCloning)/2011/09/27: Difference between revisions

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==DNA Extraction Check of Samples [G.2-1, G.2-2, G.3] and RE Digest Test==
==DNA Extraction Check of Samples [Gt.2-1, Gt.2-2, Gt.3] and RE Digest Test==
* The DNA extraction samples from 9/23/2011 were checked used the Qubit Fluorescent Quantifier for concentration values.  The G.2 samples were extremely low, but the G.3 sample was more promising at 6.88 µg/mL, so that is the one we decided to use RE digest with on an electrophoresis gel (1.0% agarose).
* The DNA extraction samples from 9/23/2011 were checked using the Qubit Fluorescent Quantifier for DNA concentration values.  The Gt.2 samples were extremely low, but the Gt.3 sample was more promising at 6.88 µg/mL, so that is the one we decided to use for the RE digest with an electrophoresis gel (1.0% agarose).  After running the gel, only the ladder showed up.  This means the DNA concentration was still too low to be seen on the gel.  For next time, we will try running a PCR with this sample and checking it on a gel for our gene.




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DNA Extraction Check of Samples [Gt.2-1, Gt.2-2, Gt.3] and RE Digest Test

  • The DNA extraction samples from 9/23/2011 were checked using the Qubit Fluorescent Quantifier for DNA concentration values. The Gt.2 samples were extremely low, but the Gt.3 sample was more promising at 6.88 µg/mL, so that is the one we decided to use for the RE digest with an electrophoresis gel (1.0% agarose). After running the gel, only the ladder showed up. This means the DNA concentration was still too low to be seen on the gel. For next time, we will try running a PCR with this sample and checking it on a gel for our gene.


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