G.tigrina Hox gene DthoxC insertion into prokaryote E.coli / (by UNIamCloning)/2011/11/17: Difference between revisions
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(Autocreate 2011/11/17 Entry for G.tigrina_Hox_gene_DthoxC_insertion_into_prokaryote_E.coli_/_(by_UNIamCloning)) |
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== | ==New Restriction Digest/Purification of Plasmid and Gel/Extraction of GFP and ATF1 == | ||
* | * Today we ran a new digest and purification of the blank plasmid (using the correct kit!!-GeneJet PCR Purification Kit). Also, we used our already digested (with KPN1 and EcoR1/Pst1 enzymes) samples of GFP and ATF1 and combined the samples into one large sample of GFP and one large sample of ATF1. Next, we set up a 1.0% gel ( two wells were taped together to make one big well for the GFP sample and the same was done for the ATF1 sample) to run the samples.... | ||
Revision as of 15:19, 17 November 2011
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New Restriction Digest/Purification of Plasmid and Gel/Extraction of GFP and ATF1
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