G.tigrina Hox gene DthoxC insertion into prokaryote E.coli / (by UNIamCloning)/2011/12/01: Difference between revisions
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== | == Transformation of Ligated GFP & ATF1 Genes in Plasmid Vectors == | ||
* | * Today, we transformed our GFP & ATF1 genes, now ligated into their plasmid vectors, into ''E.coli'' competent cells. (We used the same procedure as the previous transformation of our resuspended DNA). | ||
Once the plasmids have been taken up into the cells, plated, and allowed to grow overnight in the oven at 37°C. Tomorrow, then, we can check for colonies and should be able to see if GFP is expressing - (the bacteria will be green). After inoculation, the bacteria will hopefully have amplified themselves enough to test the expression of ATF1 - (if working, they will be producing a banana odor). | |||
Revision as of 15:23, 1 December 2011
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Transformation of Ligated GFP & ATF1 Genes in Plasmid Vectors
Once the plasmids have been taken up into the cells, plated, and allowed to grow overnight in the oven at 37°C. Tomorrow, then, we can check for colonies and should be able to see if GFP is expressing - (the bacteria will be green). After inoculation, the bacteria will hopefully have amplified themselves enough to test the expression of ATF1 - (if working, they will be producing a banana odor).
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