G.tigrina Hox gene DthoxC insertion into prokaryote E.coli / (by UNIamCloning)/2011/09/21

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(Autocreate 2011/09/21 Entry for G.tigrina_Hox_gene_DthoxC_insertion_into_prokaryote_E.coli_/_(by_UNIamCloning))
Current revision (15:16, 27 September 2011) (view source)
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==Entry title==
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==DNA Extraction and Purification from ''G.tigrinia'' 9/21/11==
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* DNA extraction using both halves of one planarian (Gt.1) was completed using the QIAGEN DNeasy blood and tissue kit. The kit calls for 20-200 µl of buffer AE. We used 35 µl Buffer AE on the assumption that our final yield of DNA would be low and to produce a higher concentration of DNA in the final sample.

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DNA Extraction and Purification from G.tigrinia 9/21/11

  • DNA extraction using both halves of one planarian (Gt.1) was completed using the QIAGEN DNeasy blood and tissue kit. The kit calls for 20-200 µl of buffer AE. We used 35 µl Buffer AE on the assumption that our final yield of DNA would be low and to produce a higher concentration of DNA in the final sample.


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