G.tigrina Hox gene DthoxC insertion into prokaryote E.coli / (by UNIamCloning)/2011/10/25
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Transformation of E.coli competent cells with resuspended GFP and ATF1 DNA
After adding 950ul - (from the protocol... next time, only add 200ul!) - SOC NP to the tubes of resuspended DNA and competent cells, we allowed them to "shake" - (mix) - for 60 minutes. Then, we spread the GFP and ATF1 mixes and our two controls onto ampicillin plates, and our negative control and leftover competent cells onto non-antibiotic plates, and placed them into the oven at 37°C overnight.