G.tigrina Hox gene DthoxC insertion into prokaryote E.coli / (by UNIamCloning)/2011/12/01

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Transformation of Ligated GFP & ATF1 Genes in Plasmid Vectors

Today, we transformed our GFP & ATF1 genes, now ligated into their plasmid vectors, into E.coli competent cells. (We used the same procedure as the previous transformation of our resuspended DNA, except using LB solution instead of SOC). Once the plasmids have been taken up into the cells, plated, and allowed to grow overnight in the oven at 37°C. Tomorrow, then, we can check for colonies and should be able to see if GFP is expressing - (the bacteria will be green). After inoculation, the bacteria will hopefully have amplified themselves enough to test the expression of ATF1 - (if working, they will be producing a banana odor).


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