G418
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| - | + | ==Experimental== | |
| + | Gentamycin analog, used to select for the KanMX marker in yeast. | ||
| + | ===Stock Solution=== | ||
| + | 200mg/mL (1000X), stored at -20 C | ||
| + | |||
| + | ===Working Concentration=== | ||
After transformation incubate culture at 30 C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L G418. | After transformation incubate culture at 30 C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L G418. | ||
| - | + | ==Mode of Action== | |
| + | Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase. | ||
| + | |||
| + | ==Safety== | ||
Revision as of 12:52, 1 August 2005
Contents |
Experimental
Gentamycin analog, used to select for the KanMX marker in yeast.
Stock Solution
200mg/mL (1000X), stored at -20 C
Working Concentration
After transformation incubate culture at 30 C for 2-3 hours and then plate on YPD containing 200 mg/L G418.
Mode of Action
Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.
