G418

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[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).
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==Experimental==
==Experimental==
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Gentamycin analog, used to select for the KanMX marker in yeast.
 
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===Stock Solution===
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* '''stock solution''': 200mg/mL (1000X), stored at -20 C
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200mg/mL (1000X), stored at -20 C
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* '''working concentration''': working concentration depends on organism and purpose of antibiotic application; stable at RT
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===Working Concentration===
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:* bacteria selection: 8-16 µg/ml
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Working concentration depends on organism and purpose of antibiotic application.
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* bacteria selection: 8-16 µg/ml
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:* plant cell maintenance: 10 µg/ml
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:* plant cell selection: 25-50 µg/ml
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* plant cell maintenance: 10 µg/ml
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:* mammalian cell maintenance: 200 µg/ml
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* plant cell selection: 25-50 µg/ml
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:* mammalian cell selection: 400 µg/ml (range: 300-1000 µg/ml)
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:* yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L (=µg/ml) G418.
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* mammalian cell maintenance: 200 µg/ml
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==Description==
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* mammalian cell selection: 400 µg/ml
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* yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L (=µg/ml) G418.
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==Mode of Action==
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* Gentamycin analog, used to select for the KanMX marker in yeast.
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* '''mode of action''': irreversibly binds to 80S ribosomal subunit, disrupting proofreading.
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Irreversibly binds to 80S ribosomal subunit, disrupting proofreading.
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* '''mode of resistance''': ''neo/kan'' gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, [[neomycin]] and [[kanamycin]] by phosphorylation.
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==Mode of resistance==
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''neo/kan'' gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, [[neomycin]] and [[kanamycin]] by phosphorylation.
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==Safety==
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==Links==
==Links==
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* related antibiotics: [[kanamycin]], [[neomycin]] (all aminoglycosides)
* FAQ on G418 [http://www.g418.com/faq.htm]
* FAQ on G418 [http://www.g418.com/faq.htm]
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* list of cell culture material by company [http://www.krackeler.com/products/fid/2910]
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[[Category:Material]] [[Category:Antibiotic]]

Current revision

Aminoglycoside antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).

Experimental

  • stock solution: 200mg/mL (1000X), stored at -20 C
  • working concentration: working concentration depends on organism and purpose of antibiotic application; stable at RT
  • bacteria selection: 8-16 µg/ml
  • plant cell maintenance: 10 µg/ml
  • plant cell selection: 25-50 µg/ml
  • mammalian cell maintenance: 200 µg/ml
  • mammalian cell selection: 400 µg/ml (range: 300-1000 µg/ml)
  • yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on YPD containing 200 mg/L (=µg/ml) G418.

Description

  • Gentamycin analog, used to select for the KanMX marker in yeast.
  • mode of action: irreversibly binds to 80S ribosomal subunit, disrupting proofreading.
  • mode of resistance: neo/kan gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin and kanamycin by phosphorylation.

Links

  • related antibiotics: kanamycin, neomycin (all aminoglycosides)
  • FAQ on G418 [1]
  • list of cell culture material by company [2]
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