G418

Experimental

• stock solution: 200mg/mL (1000X), stored at -20 C
• working concentration: working concentration depends on organism and purpose of antibiotic application; stable at RT

• bacteria selection: 8-16 µg/ml

• plant cell maintenance: 10 µg/ml
• plant cell selection: 25-50 µg/ml

• mammalian cell maintenance: 200 µg/ml
• mammalian cell selection: 400 µg/ml (range: 300-1000 µg/ml)
• yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on YPD containing 200 mg/L (=µg/ml) G418.

Description

• Gentamycin analog, used to select for the KanMX marker in yeast.
• mode of action: irreversibly binds to 80S ribosomal subunit, disrupting proofreading.
• mode of resistance: neo/kan gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin and kanamycin by phosphorylation.

Links

• FAQ on G418 [1]
• list of cell culture material by company [2]