GFP

• wtGFP on NCBI

GFP Variants

• GFPmut3b - S65G, S72A
• GFPmut3* - mutations from wt: S2R,S65G,S72A
  • This is GFPmut3b[1] with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors.

References

1. Cormack BP, Valdivia RH, and Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 1996;173(1 Spec No):33-8. DOI:10.1016/0378-1119(95)00685-0 |

Molecular Biology and Muation of Green Fluorescent Protein (Book Chapter, Zacharias & Tsien)