GHV-68 Plaque Assay

(Difference between revisions)

Revision as of 15:39, 30 May 2007

Plaque assay for gHV-68 using 3T12 fibroblasts. Stolen from Virgin lab protocols.

Methylcellulose
X μL reagent 2
- component A (reagent 2 is made up of multiple components)
- component B
equipment 1
equipment 2

Day 0

Trypsinize 3T12's (1 confluent T175 will yield approx. 10-40x10^6, cells) and resuspend at 2.5-2.5x10⁶/ml in DMEM-10.
Aliquot 2/ml/well (3-5x10⁵ cells/well) into 6-well plates. Don't swirl the cells, as you may end up with all your 3T12's in the center of the well.
1. Need one 6-well plate per viral sample. (This will let you test 6 logs of dilution)
Prepare methylcellulose, if necessary.
Freeze/thaw/freeze your viral sample, if necessary.

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@@ Line 17: / Line 17: @@
 #Aliquot 2/ml/well (3-5x10<sup>5</sup> cells/well) into 6-well plates. Don't swirl the cells, as you may end up with all your 3T12's in the center of the well.
 ##Need one 6-well plate per viral sample. (This will let you test 6 logs of dilution)
-#Prepare [[Methylcellulose]], if necessary.
+#Prepare [[methylcellulose]], if necessary.
 #Freeze/thaw/freeze your viral sample, if necessary.