GHV-68 Plaque Assay

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Overview

Plaque assay for gHV-68 using 3T12 fibroblasts. Stolen from Virgin lab protocols.

Materials

• Methylcellulose
• X μL reagent 2
  • component A (reagent 2 is made up of multiple components)
  • component B
• equipment 1
• equipment 2

Procedure

Day 0

1. Trypsinize 3T12's (1 confluent T175 will yield approx. 10-40x10</sup>6
2. Incubate at 37° C for 20-30 min.
3. Add 0.1 volume resuspended DNase Inactivation Reagent.
4. Incubate 2 min at RT mixing every 30s.
5. Centrifuge at 10,000xg for 1.5 min.
6. Transfer solution to a new tube avoiding pelleted Inactivation Reagent.

Notes

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References

Relevant papers and books

1. Goldbeter A and Koshland DE Jr. . pmid:6947258. PubMed HubMed [Goldbeter-PNAS-1981]
2. JACOB F and MONOD J. . pmid:13718526. PubMed HubMed [Jacob-JMB-1961]
3. Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. isbn:0879697164. [Ptashne-Genetic-Switch]

Contact

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