GYT

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Summary

GYT Medium. Used in preparing electrocompetent cells.

Ingredients

  • 10% (v/v) glycerol
  • 0.125% (w/v) yeast extract
  • .25% (w/v) tryptone

Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Store in 2.5-ml aliquots at 4°C.

From Molecular Cloning

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