Gama:Lab Manual

Basic information for Lab-members

Institutional setting

The RNA Biology and Bioinformatic group was established in 2009 and is part of the Gene Expression and Bioinformatics Unit of the FCT funded Research Center for Biodiversity, Functional and Integrative Genomics (BioFIG). The group is also affiliated to the Department of Chemistry and Biochemistry, Faculty of Sciences of Lisbon and the lab facilities are located within this department.

BioFIG is composed of a total of 5 research Units, with most of its research teams located at the Faculty of Sciences or at the National Health's Institute Dr. Ricardo Jorge (INSA-RJ), within walking distance of the University campus.

The Gene Expression and Bioinformatics Unit additionally includes the RNA metabolism group (PI Luisa Romão) and the Signalling in Gene Expression group (PI Peter Jordan), based at INSA-RJ.

BioFIG project and financial management (including fellowships and contracts) are performed by a private Foundation called Fundação da Faculdade de Ciências (FFCUL).

Facilities

Main working facilities are located in the C8 building:

Main lab: Room 8.5.56 - shared with the MPD Unit

Bioinformatics Lab: Room 8.2.41

Margarida's Office: Room 8.2.53 (shared with Prof. M. Telhada)

Storage room and fluorescence microscope: Room 8.5.56b (access from the main lab) - shared with the MPD Unit

Cell culture lab: Room 8.1.74 - shared with the MPD Unit

Supporting labs (equipment and radioactive room): Rooms 8.1.75/78 - MPD Unit

In the 4th floor (Biochemistry Dept) there are several common facilities:

-80 Freezers and Ice dispenser

Cold Room

Dark-room

Water and autoclaving

Preparative Centrifuges

Other facilities frequently used facilities:

Real time PCR - C2 building, room (Prof. Manuela Cordeiro)

BioFIG Microscopy Room - C2 building

The Microbiology Unit of BioFIG headed by Prof. Rogério Tenreiro is located at the ICAT/TechLabs building and has several relevant equipments that may be used by our group upon request (eg. centrifuges, FACS, fluorescence/bioluminescence imaging of whole animals, etc.)

Lab access and keys

A key to the RNA or Bioinformatics lab will be given to new members. This key must be returned when the lab member leaves the group.

Spare keys to the Bioinformatics lab, Real-Time PCR lab and key-locker for the supporting labs (located in floor 1. next to room 8.1.78) are kept in the lab.

A spare key to the main lab is kept in the Bioinformatics lab.

Access to the C1/C8 building is unrestricted during normal working days/hours. For access outside these periods the following form needs to be filled, signed by Margarida and delivered to the DQB secretary.

Out-of-hours access to other campus buildings depend on special permissions that need to be arranged when required.

Car-parking

Parking at the campus requires payment of an annual fee of ~70€.

The parking card can be requested at the Technical services (room 5.01.04) by presenting a declaration from the PI and the personal ID card.

Email account and internet access

A personal email account (@fc.ul.pt) can be requested online at www.fc.ul.pt.

Click on Login, select the new account option at the bottom and fill in the form.

The campus is covered by the wireless eduroam network service. Any person with an email account from a portuguese university (ul.pt) can use this wireless access.

LAN access from personal computers/Laptops requires activation of a specific network connection with the computer's mac-address.

This can be done online by the person to whom the connection is registered (most likely the PI).

Contact "Centro de Informática" for any related topic.

Lab websites

RNA Biology and Bioinformatics Website: http://openwetware.org/wiki/Gama

    - this is a Wiki Website hosted by OpenWetWare. Lab members are requested to register as OWW users to be able to modify website contents.

In addition, there are two closed google sites for sharing project and research data (links available through the main website). Lab members should ask the PI/site manager to register them as authorized users:

RNA Biology and Bioinformatics site: https://sites.google.com/site/rnabioinformatics/

Merck/HIV project site: https://sites.google.com/site/smallrnaandhiv/

Other institutional websites:

BioFIG main Website: http://biofig.fc.ul.pt/

FCUL/campus website: http://www.fc.ul.pt/

Lab note-books and stationary

Paper lab note-books and other stationary are available to lab members at the lab.

Data registered on lab books belongs to the lab and must be left behind when leaving.

The use of digital lab-books is strongly supported, even in parallel with conventional paper lab note-books.

Phones

Phones can be used for work related purposes as follows:

- for contacts within the UL campus (including IMM, the Sta Maria Hospital, Rectorate and other Faculties), just dial the 5 digit extension

- to contact numbers in the Lisbon area (area code 21), dial 0 to get an external line.

- calls outside of the Lisbon area are blocked.

For personal use of phones, a paying account with password can be requested from the Technical Services to dial from any phone on campus.

To receive calls in most phones, call 217500000 and ask the operator to connect to the extension.

Some phones have direct outside numbers.

On Campus facilities

ATM machines: C8 and C3 buildings.

Cafeterias: C8, C1, C5, C6 buildings (with lunch service); C2 building (just sandwiches).

Bookstore, stationary and gifts: Livraria Escolar Editora, C5 building.

Copy service: C4 building, ground level

Important contacts

BioFIG Secretary: Renata Vincent Room 8.2.41

MPD Unit Lab-manager: Marta Palma

Room 8.1.75, Phone: 28175

Department secretary: Cláudia Azevedo,

Room 8.6., Phone: 28632

Fundação da Faculdade de Ciências da Universidade de Lisboa Atendimento 2.ª a 6.ª feira das 9:30 h. às 12:30 h. e das 14:00 h. às 16:30 h. Campus da Faculdade de Ciências da UL Edifício C 1 - 3.º Piso Campo Grande 1749-016 Lisboa Tel.: 21 750 00 32

NIF: 503183504

Fellowships and contracts Rafael Moreira rafael.moreira@fc.ul.pt Ext.: 21 359

Orders Natalia Rodrigues requisicoes.ffcul@fc.ul.pt Ext: Ext.: 21321

Campus Security

C5 building

  * 217 500 000
  * 25205

Technical services

DesignacaoGABINETE DE INFRAESTRUTURAS E APOIO TÉCNICO ResponsávelJOSÉ FERNANDES VIDEIRA LOUSA MoradaCampo Grande, Edifício C4, Piso 1, 1749-016 - Lisboa EdificioC4 Piso1 Sala5.1.04 ATENDIMENTO Horário 09h00-17h00 Telefone(s)

  * 217 500 099
  * 24135

Fax217 500 157

Campus directory available at: http://www.fc.ul.pt/diretorio

Benefits

FFCUL fellows are offered a 24h personal insurance, including accidents outside the lab.