Gaussian Fitting for Resolution: Difference between revisions
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*[http://www.openwetware.org/images/e/e8/Gauss2dwxy.m gauss2dwxy.m] | *[http://www.openwetware.org/images/e/e8/Gauss2dwxy.m gauss2dwxy.m] | ||
*[http://www.openwetware.org/images/e/e5/Initialguess.m initialguess.m] | *[http://www.openwetware.org/images/e/e5/Initialguess.m initialguess.m] | ||
Scholars, | |||
Two Matlab functions – gaussfit and plotgaussfit – will be of extreme utility in estimating the resolution of your microscope from the PSD slide. The m-files for these functions have been placed in the fluorescence microscopy lab folder. Documentation is available at: [http://www.cellmigration-gateway.com/resource/imaging/icsmatlab/ICSTutorial.html]. | |||
The best approach might be to fit several objects and take an average. Please report the uncertainty in your estimate. | |||
--Steve | |||
Many thanks to David Kolin, the author of this excellent code. | |||
Latest revision as of 22:45, 4 November 2008
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Scholars,
Two Matlab functions – gaussfit and plotgaussfit – will be of extreme utility in estimating the resolution of your microscope from the PSD slide. The m-files for these functions have been placed in the fluorescence microscopy lab folder. Documentation is available at: [1].
The best approach might be to fit several objects and take an average. Please report the uncertainty in your estimate.
--Steve
Many thanks to David Kolin, the author of this excellent code.
