Gavage error estimation - Revision history

Jakob Suckale: illustration

2010-02-05T13:46:14Z

illustration

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		+	[[Image:Gavage needle 1ml syringe.JPG\|thumb\|right\|Gavage needle and 1ml syringe used for the error estimation plus a 30µl aliquot in a 1.5ml tube]]
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	'''Gavage''' is a commonly used method of oral administration to mice preferable to the more stressful avenue of administration via injection but worse than administration through drinking water or chow. The latter, however, is problematic if precise dosage is required.		'''Gavage''' is a commonly used method of oral administration to mice preferable to the more stressful avenue of administration via injection but worse than administration through drinking water or chow. The latter, however, is problematic if precise dosage is required.

Jakob Suckale at 13:43, 5 February 2010

2010-02-05T13:43:25Z

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	Method: The mass of 5 gavages (30 or 100µl) was measured and the error calculated. The measurement was repeated twice.		Method: The mass of 5 gavages (30 or 100µl) was measured and the error calculated. The measurement was repeated twice.
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Jakob Suckale at 13:42, 5 February 2010

2010-02-05T13:42:42Z

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	[[Category:Method]]		[[Category:Method]]
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Jakob Suckale: categories

2010-02-05T13:41:27Z

Jakob Suckale: * page created *

2010-02-05T13:40:24Z

*** page created ***

New page

'''Gavage''' is a commonly used method of oral administration to mice preferable to the more stressful avenue of administration via injection but worse than administration through drinking water or chow. The latter, however, is problematic if precise dosage is required.

For our experimental purposes we '''estimated the error''' of gavaging liquids as follows:

* '''~2µl per aliquot''' for both 30 and 100µl aliquots

The relative error scales favourably with aliquot size:

* '''7% for 30µl''' aliquots, '''2% for 100µl''' aliquots

Method: The mass of 5 gavages (30 or 100µl) was measured and the error calculated. The measurement was repeated twice.