Generating antibodies
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* [[Protein purification tags]] | * [[Protein purification tags]] | ||
* [[Western blot]] | * [[Western blot]] | ||
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| + | == External links == | ||
| + | * [http://www.hopkins-arthritis.org/physician-corner/education/mono_anti.html Introduction to monoclonal antibody generation] by Mark Soloski, Johns Hopkins | ||
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| + | Wikipedia | ||
| + | * [[wikipedia:Antibody|Antibody]], esp. [[wikipedia:Antibody#Research_applications|research applications]] | ||
| + | * [[wikipedia:Polyclonal antibodies|Polyclonal antibodies]], [[wikipedia:Monoclonal antibodies|Monoclonal antibodies]] | ||
Revision as of 10:54, 22 April 2008
You are hot in pursuit of an interesting protein but there are no commercial antibodies or they are bad. It's time to think about raising your own antibodies against the protein. It will be a time and money consuming undertaking but once you developed a specific antibody or set of antibodies, you will be in a unique position to generate interesting data.
Contents |
Early decisions
Type of antibody
Source organism
Type of antigen to use
See also
External links
- Introduction to monoclonal antibody generation by Mark Soloski, Johns Hopkins
Wikipedia
