Generating antibodies

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== External links ==
== External links ==
* [http://www.hopkins-arthritis.org/physician-corner/education/mono_anti.html Introduction to monoclonal antibody generation] by Mark Soloski, Johns Hopkins
* [http://www.hopkins-arthritis.org/physician-corner/education/mono_anti.html Introduction to monoclonal antibody generation] by Mark Soloski, Johns Hopkins
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* [http://mabs.unc.edu/services.htm Hybridoma and in vitro generation of antibodies], Antibody Facility, UNC Chapel Hill
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Protocol Online (methods forum)
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* [http://www.protocol-online.org/cgi-bin/htsearch?config=htdig_pol&restrict=&exclude=&words=antibody Protocol-online.org archive, keyword antibody] and [http://www.protocol-online.org/biology-forums/antibody.html discussions indexed under antibody]
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* [http://www.protocol-online.org/biology-forums/posts/29480.html Affinity purification]
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Wikipedia
Wikipedia

Revision as of 11:13, 22 April 2008

You are hot in pursuit of an interesting protein but there are no commercial antibodies or they are bad. It's time to think about raising your own antibodies against the protein. It will be a time and money consuming undertaking but once you developed a specific antibody or set of antibodies, you will be in a unique position to generate interesting data.

Contents

Early decisions

Type of antibody

Source organism

Type of antigen to use

See also

External links

Protocol Online (methods forum)

Wikipedia

Personal tools