Generating antibodies

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[[Image:Antibody schematic.jpg|thumb|right|200px|Drawing of an antibody - heavy chain red, light chain yellow.]]
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You are hot in pursuit of an interesting protein but there are no commercial antibodies or they are bad. It's time to think about raising your own antibodies against the protein. It will be a time and money consuming undertaking but once you developed a specific antibody or set of antibodies, you will be in a unique position to generate interesting data.
You are hot in pursuit of an interesting protein but there are no commercial antibodies or they are bad. It's time to think about raising your own antibodies against the protein. It will be a time and money consuming undertaking but once you developed a specific antibody or set of antibodies, you will be in a unique position to generate interesting data.

Revision as of 10:27, 22 April 2008

Drawing of an antibody - heavy chain red, light chain yellow.
Drawing of an antibody - heavy chain red, light chain yellow.

You are hot in pursuit of an interesting protein but there are no commercial antibodies or they are bad. It's time to think about raising your own antibodies against the protein. It will be a time and money consuming undertaking but once you developed a specific antibody or set of antibodies, you will be in a unique position to generate interesting data.

Contents

Early decisions

Type of antibody

Source organism

Type of antigen to use

See also

External links

Protocol Online (methods forum)

Wikipedia

Personal tools