Genomic Miniprep: Difference between revisions
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(New page: This procedure is identical to the normal miniprep procedure except the step in red, and less culture is prepped<br> 1. Pellet 1.5 mL saturated culture by spinning full speed, 30 seconds.<...) |
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Latest revision as of 17:15, 15 June 2010
This procedure is identical to the normal miniprep procedure except the step in red, and less culture is prepped
1. Pellet 1.5 mL saturated culture by spinning full speed, 30 seconds.
2. Add 250uL of P1 buffer into each tube. Resuspend the cells using
a vortexer.
3. Add 250uL of 2% SDS (stored in a 50 mL conicol) (actually stored in falcon tube. Also, this step replaces the P2 step.
4. Incubate at 55 degrees until clear (roughly 15-20min?).
5. Continue as usual with N3 step.
