http://www.openwetware.org/index.php?title=Genomic_Miniprep&feed=atom&action=history 2013-05-22T05:35:11Z Revision history for this page on the wiki MediaWiki 1.13.2 http://www.openwetware.org/index.php?title=Genomic_Miniprep&diff=424417&oldid=prev 2010-06-16T00:15:12Z

<p>New page: This procedure is identical to the normal miniprep procedure except the step in red, and less culture is prepped&lt;br&gt; 1. Pellet 1.5 mL saturated culture by spinning full speed, 30 seconds.&lt;...</p> <p><b>New page</b></p><div>This procedure is identical to the normal miniprep procedure except the step in red, and less culture is prepped&lt;br&gt;<br /> 1. Pellet 1.5 mL saturated culture by spinning full speed, 30 seconds.&lt;br&gt;<br /> 2. Add 250uL of P1 buffer into each tube. Resuspend the cells using<br /> a vortexer.&lt;br&gt;<br /> 3. Add 250uL of 2% SDS (stored in a 50 mL conicol) (actually stored in falcon tube. Also, this step replaces the P2 step. &lt;br&gt;<br /> 4. Incubate at 55 degrees until clear (roughly 15-20min?). &lt;br&gt;<br /> 5. Continue as usual with N3 step. &lt;br&gt;</div>

Tahoura Samad