Genomic Miniprep

From OpenWetWare

Revision as of 20:15, 15 June 2010 by Tahoura Samad (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

This procedure is identical to the normal miniprep procedure except the step in red, and less culture is prepped
1. Pellet 1.5 mL saturated culture by spinning full speed, 30 seconds.
2. Add 250uL of P1 buffer into each tube. Resuspend the cells using a vortexer.
3. Add 250uL of 2% SDS (stored in a 50 mL conicol) (actually stored in falcon tube. Also, this step replaces the P2 step.
4. Incubate at 55 degrees until clear (roughly 15-20min?).
5. Continue as usual with N3 step.

Personal tools