Gerber:Resources: Difference between revisions
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* '''[[Media:Affinity purification of yeast ribosomes.pdf|Yeast Ribosome Purification Protocol]]''' {{hide| | * '''[[Media:Affinity purification of yeast ribosomes.pdf|Yeast Ribosome Purification Protocol]]''' {{hide| | ||
Immunopurification protocol for the pulldown of tagged ribosomal proteins and the co-purification of (associated) RNA molecules from '''yeast''' cell extracts. RNA prepared according to this protocol can be analyzed using microarrays or (possibly) sequencing techniques. | Immunopurification protocol for the pulldown of tagged ribosomal proteins and the co-purification of (associated) RNA molecules from '''yeast''' cell extracts. RNA prepared according to this protocol can be analyzed using microarrays or (possibly) sequencing techniques. |
Revision as of 00:59, 5 May 2009
ProtocolsImmunopurification protocol for the pulldown of endogenous RNA-binding proteins ("RIP") and co-purification of (associated) RNA molecules from human cell extracts. RNA prepared according to this protocol can be analyzed using microarrays or (possibly) sequencing techniques, allowing the identification of the RNA targets of the RBP under study.
This protocol has been optimized for the immunopurification of endogenous human PUM1 and PUM2 proteins from HeLa S3 cells.
Immunopurification protocol for the pulldown of tagged ribosomal proteins and the co-purification of (associated) RNA molecules from yeast cell extracts. RNA prepared according to this protocol can be analyzed using microarrays or (possibly) sequencing techniques.
This protocol has been optimized for the study of yeast ribosomes through the immunopurification of the large ribsomal subunit protein Rpl16a.
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