Gerber:Resources
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== Protocols == | == Protocols == | ||
| - | * '''[[Media:Galgano_RIP_protocol.pdf|RIP Protocol]]''' | + | * '''[[Media:Galgano_RIP_protocol.pdf|Human RIP Protocol]]''' |
| - | Immunopurification protocol for the pulldown of '''endogenous''' RNA-binding proteins ("RIP") and co-purification of (associated) RNA molecules from '''human''' cell extracts. RNA prepared according to this protocol can be analyzed using microarrays or sequencing techniques, allowing the identification of the RNA targets of the RBP under study. | + | Immunopurification protocol for the pulldown of '''endogenous''' RNA-binding proteins ("RIP") and co-purification of (associated) RNA molecules from '''human''' cell extracts. RNA prepared according to this protocol can be analyzed using microarrays or (possibly) sequencing techniques, allowing the identification of the RNA targets of the RBP under study.<br/><br/> |
| - | This protocol has been optimized for the immunopurification of endogenous human PUM1 and PUM2 proteins from HeLa S3 cells.<br/> | + | This protocol has been optimized for the immunopurification of endogenous human PUM1 and PUM2 proteins from HeLa S3 cells.<br/><br/> |
| - | Protocol | + | Protocol by '''Alessia Galgano'''.<br/><br/> |
| - | <u> | + | <u>References:</u> |
<biblio> | <biblio> | ||
| - | # | + | #Original_article pmid=18776931 |
| + | #Method pmid=21431753 | ||
| + | </biblio> | ||
| + | |||
| + | <br/><br/> | ||
| + | |||
| + | * '''[[Media:Affinity purification of yeast ribosomes.pdf|Yeast Ribosome Purification Protocol]]''' | ||
| + | Immunopurification protocol for the pulldown of tagged ribosomal proteins and the co-purification of (associated) RNA molecules from '''yeast''' cell extracts. RNA prepared according to this protocol can be analyzed using microarrays or (possibly) sequencing techniques.<br/><br/> | ||
| + | This protocol has been optimized for the study of yeast ribosomes through the immunopurification of the large ribsomal subunit protein Rpl16a.<br/><br/> | ||
| + | Protocol by '''Regula Halbeisen'''.<br/><br/> | ||
| + | <u>References:</u> | ||
| + | <biblio> | ||
| + | #Original_article pmid=19419242 | ||
| + | #Method pmid=19398006 | ||
</biblio> | </biblio> | ||
| - | |||
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ProtocolsImmunopurification protocol for the pulldown of endogenous RNA-binding proteins ("RIP") and co-purification of (associated) RNA molecules from human cell extracts. RNA prepared according to this protocol can be analyzed using microarrays or (possibly) sequencing techniques, allowing the identification of the RNA targets of the RBP under study.
Immunopurification protocol for the pulldown of tagged ribosomal proteins and the co-purification of (associated) RNA molecules from yeast cell extracts. RNA prepared according to this protocol can be analyzed using microarrays or (possibly) sequencing techniques.
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