Getting started on the microscope: Difference between revisions
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This is tutorial (in development) for getting started on the microscope. | This is tutorial (in development) for getting started on the microscope. | ||
== | == How to sign up for the scope== | ||
*We have a Google calendar for scope usage: | |||
[http://www.google.com/calendar/render?cid=ko76k8i0j3jd4p4f2i4lebho64%40group.calendar.google.com http://www.google.com/calendar/images/ext/gc_button1_en.gif] | |||
==How to turn on the scope== | |||
(order doesn't matter) | |||
#Turn on FL lamp | |||
#*takes 2 minutes to warm up | |||
#*takes 30 minutes to cool down (so don't start again in <30 min) | |||
#Turn on transmitted light lamp | |||
#Turn on scope (switch is on back left of the base) | |||
#Turn on Ludl Box (controls shutters) | |||
#Turn on camera | |||
==How do I find my cells?== | ==How do I find my cells?== | ||
==Common | |||
If this doesn't work then you may need to [[Focusing the Nikon TE2000 Condenser|focus the condenser]], this is a little tricky so you should be shown how to do it by [[Jason Kelly|jason]] first. | |||
==Common Problems== | |||
===I don't see anything in the eyepiece=== | |||
#The optical path is not pointing to the eyepiece, you can change it on the control pad. | |||
===The fine focus knob doesn't move the turret=== | |||
Two possible problems: | |||
#(more likely) You have turned on the fine focus knob which is seperate from the base. Only one can control the turret at a given time, turn off the satellite knob. | |||
#The z-stage is too low (<0 position as read from control pad) or too high (>6000? need to check this.). Turn the scope off and on and you should be OK. |
Latest revision as of 10:04, 1 February 2007
This is tutorial (in development) for getting started on the microscope.
How to sign up for the scope
- We have a Google calendar for scope usage:
http://www.google.com/calendar/images/ext/gc_button1_en.gif
How to turn on the scope
(order doesn't matter)
- Turn on FL lamp
- takes 2 minutes to warm up
- takes 30 minutes to cool down (so don't start again in <30 min)
- Turn on transmitted light lamp
- Turn on scope (switch is on back left of the base)
- Turn on Ludl Box (controls shutters)
- Turn on camera
How do I find my cells?
If this doesn't work then you may need to focus the condenser, this is a little tricky so you should be shown how to do it by jason first.
Common Problems
I don't see anything in the eyepiece
- The optical path is not pointing to the eyepiece, you can change it on the control pad.
The fine focus knob doesn't move the turret
Two possible problems:
- (more likely) You have turned on the fine focus knob which is seperate from the base. Only one can control the turret at a given time, turn off the satellite knob.
- The z-stage is too low (<0 position as read from control pad) or too high (>6000? need to check this.). Turn the scope off and on and you should be OK.